Spectroscopic analysis of mushrooms by surface-enhanced Raman scattering (SERS)

Abstract Background Mushrooms have always been considered an important source of food and biologically active compounds with several medicinal properties. In recent years, different methods were used to study the quality chemical composition mushrooms. Among these, Fourier transform infrared (FT-IR) FT-Raman spectroscopy techniques successfully applied identify mushroom species. However, structural biomolecule components existing in mycelium or fruiting bodies may produce strong fluorescence emission that overlaps Raman radiation, thus avoiding their analyses by Raman. SERS is a powerful technique which uses metal nanoparticles (NPs) enhance signal molecules adsorbed on NPs surface. addition, able quench macromolecule florescence. this work, we employed silver order get fingerprints based as quick procedure analyze from six edible and/or mushrooms: Lentinula edodes , Ganoderma lucidum Pleurotus cornucopiae ostreatus Tuber aestivum magnatum . Results performed directly body fragments produced characteristic spectra for each One group mushrooms ( L. G. T. ) was dominated bands nucleic acids; other one P. ), pigments such melanins; carotenoids; azafilones; polyketides; flavonoids located cell wall. Additionally, corresponding wall polysaccharides, particularly chitosan 1,3-? D-glucan, identified extracts No polysaccharides found extract. mapping analyzed samples useful tracking spatial distribution marker bands. Moreover, principal component analysis (PCA) carried out acquired spectra, allows discriminate Conclusions The has ability generate using Ag-NPs deposited intact, untreated tissues. Using methodology, commonly laboratory time-consuming can be avoided bypassed well time reduced. Graphical